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KMID : 0545120040140061333
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Journal of Microbiology and Biotechnology
2004 Volume.14 No. 6 p.1333 ~ p.1337
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The Effect of Overexpression of Rat Clusterin in L929 Fibroblasts
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Park JH
Ju SK/Park JS/Park YK/Kang MH/You KH
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Abstract
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Oxidants such as hydrogen peroxide are powerful inducers of cell damage ageing and apoptosis. Since clusterin a 75- 80 kDa mammalian glycoprotein is frequently found to be inducible in apoptotic cells and tissues this study inquired into whether this would be a protective mechanism against further cell death. The aim was to find out whether overexpression of clusterin could protect cells from oxidantinduced stress and apoptosis. To clarify this issue we generated and analyzed stable cell lines expressing fusion proteins of a rat clusterin with an enhanced green fluorescent protein (EGFP). When treated with varying concentrations of hydrogen peroxides clusterin transfectants indeed showed increased resistance to apoptosis and exhibited a much higher survival rate than mock-transfected cells. On the other hand neither intracellular re-distribution nor local concentration of clusterin-EGFP was observed which leaves the question open about its anti-apoptotic mechanism. In conclusion the overexpression of clusterin provides a means for protecting cells against oxidative stress and subsequent cell death.
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